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This combination is then incubated at 55°C for just one hour. Then the sample is phenol-extracted when with phenol/chloroform/isoamyl Alcoholic beverages Remedy and centrifuged. The aqueous layer is eradicated into a fresh microcentrifuge tube. The DNA is ethanol-precipitated, re-suspended in buffer, and ethanol-precipitated a 2nd time. Once the p